We invented a multiplexable super-resolution force spectroscopy that can resolve intermolecular bonds, reveal molecular motion, and distinguish multiple-step protein binding. The super-resolution force spectroscopy (SURFS) technique uses precise ultrasound radiation to exert acoustic radiation force on protein bonds labeled with magnetic particles; the force-induced dissociation will be measured as a reduced magnetic signal by a magnetic sensor, such as an atomic magnetometer. The force resolution reaches ~0.5 pN (piconewton), which is approximately one order of magnitude better than all the current force spectroscopy techniques. The associated spatial resolution for molecular motion is ~0.2 nm, which cannot be revealed by structural techniques such as X-ray crystallography and cryo-electron microscopy. With a scheme of differential force fields, time resolution of ~1-3 min can be achieved, allowing the resolution of the multiple steps of protein binding.