Development of soft and conductive microstructures is of great interest in various fields including electronics, optics, and bioscience. Among various fabrication methods, two-photon polymerization (TPP) based on direct laser writing is a promising 3D printing technique for construction of structures in submicron resolution, using a wide range of photocurable inks. Herein, we introduce a novel resin which can be fabricated into conductive and bioactive microstructures via TPP. For the first time, we report that direct incorporation of conductive poly (3,4-ethylenedioxythiophene)poly( styrenesulfonate) (PEDOT:PSS) in a poly(ethylene glycol) diacrylate (PEGDA)-based blend remarkably enhances the electrical conductivity of microstructures over 10 orders of magnitude. Moreover, presence of laminin protein encourages cellular adhesion to the microstructures in vitro. Further, we have developed high performance fully functional biosensors made of hybrid nonconductive and conductive structures for sensitive detection of glucose by functionalization of the ink with enzyme glucose oxidase. Our novel Conductive ink opens new avenues for development cost-effective microelectronics in a broad range of biomedical research, electronics and sensors.